Decree No. 497 / 2004 Coll.
Decree amending Decree of the Ministry of Agriculture No. 124 / 2001 Coll., laying down the requirements for sampling and the principles of methods of analysis of feedingstuffs, additives and premixtures and the method of storage of samples
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497
DECLARATION
of 10 September 2004
amending Decree No. 124 / 2001 of the Ministry of Agriculture Coll., laying down the sampling requirements and principles for methods of analysis of feedingstuffs, additives and premixtures and the method of storage of samples
The Ministry of Agriculture provides, pursuant to § 17 paragraphs 8 and 9 of Act No. 91 / 1996 Coll., on Feed, as amended by Act No. 244 / 2000 Coll., Act No. 147 / 2002 Coll., Act No. 320 / 2002 Coll. and Act No. 21 / 2004 Coll., hereinafter "the Act ':
Decree No. 124 / 2001 Coll., laying down the sampling requirements and principles of methods of analysis for feed, feed additives and premixtures and the method of storage of samples, shall be amended as follows:
1. Paragraph 1 (1) and (2), including footnotes 1 and 1a, read:
"(1) This decree (1) incorporates the relevant provisions of the European Communities (1a) and regulates the requirements for sampling, the principles of methods of analysis of feed, additives and premixtures and the method of storage of samples.
(2) When sampling feed, additives and premixtures carried out in the framework of professional supervision and testing (Sections 16 to 19 of the Act), the procedures set out in this Decree shall apply.
Paragraph 2 shall become paragraph 3.
1) It is issued on the basis and within the limits of the law, the content of which allows the relevant provisions of the European Communities to be incorporated by decree.
(1a) Council Directive 70 / 373 / EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feedingstuffs. Council Directive 72 / 275 / EEC of 20 July 1972 amending the Directives on the introduction of Community methods of sampling and analysis for the official control of feedingstuffs. First Commission Directive 71 / 250 / EEC of 15 June 1971 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 81 / 680 / EEC of 30 July 1981 amending Directives 71 / 250 / EEC, 71 / 393 / EEC, 72 / 199 / EEC, 73 / 46 / EEC, 74 / 203 / EEC, 75 / 84 / EEC, 76 / 372 / EEC and 78 / 633 / EEC concerning the establishment of Community methods of analysis for the official control of feedingstuffs. Commission Directive 98 / 54 / EC of 16 July 1998 amending Directives 71 / 250 / EEC, 72 / 199 / EEC, 73 / 46 / EEC and repealing Directive 75 / 84 / EEC. Commission Directive 1999 / 27 / EC of 20 April 1999 laying down Community methods of analysis for the determination of amprolium, diclazuril and carbadox in feedingstuffs, amending Directives 71 / 250 / EEC and 73 / 46 / EEC and repealing Directive 74 / 203 / EEC. Second Commission Directive 71 / 393 / EEC of 18 November 1971 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 73 / 47 / EEC of 5 December 1972 amending the second Commission Directive of 18 November 1971 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 81 / 680 / EEC of 30 July 1981 amending Directives 71 / 250 / EEC, 71 / 393 / EEC, 72 / 199 / EEC, 73 / 46 / EEC, 74 / 203 / EEC, 75 / 84 / EEC, 76 / 372 / EEC and 78 / 633 / EEC concerning the establishment of Community methods of analysis for the official control of feedingstuffs. Commission Directive 84 / 4 / EEC of 20 December 1983 amending Directives 71 / 393 / EEC, 72 / 199 / EEC and 78 / 633 / EEC concerning the determination of Community methods of analysis for the official control of feedingstuffs. Commission Directive 98 / 64 / EC of 3 September 1998 laying down Community methods of analysis for the determination of amino acids, fats and olaquindox in feedingstuffs and amending Directive 71 / 393 / EEC. Third Commission Directive 72 / 199 / EEC of 27 April 1972 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 92 / 89 / EEC of 3 November 1992 amending Annex I to fourth Directive 73 / 46 / EEC laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 98 / 54 / EC of 16 July 1998 amending Directives 71 / 250 / EEC, 72 / 199 / EEC, 73 / 46 / EEC and repealing Directive 75 / 84 / EEC. Commission Directive 1999 / 27 / EC of 20 April 1999 laying down Community methods of analysis for the determination of amprolium, diclazuril and carbadox in feedingstuffs, amending Directives 71 / 250 / EEC and 73 / 46 / EEC and repealing Directive 74 / 203 / EEC. Fourth Commission Directive 73 / 46 / EEC of 5 December 1972 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 92 / 89 / EEC of 3 November 1992 amending Annex I to fourth Directive 73 / 46 / EEC laying down Community methods of analysis for the official control of feedingstuffs. First Commission Directive 76 / 371 / EEC of 1 March 1976 laying down Community methods of sampling for the official control of feedingstuffs. Seventh Commission Directive 76 / 372 / EEC of 1 March 1976 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 94 / 14 / EC of 29 March 1994 amending seventh Directive 76 / 372 / EEC laying down Community methods of analysis for the official control of feedingstuffs. Eighth Commission Directive 78 / 633 / EEC of 15 June 1978 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 81 / 715 / EEC of 31 July 1981 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 84 / 425 / EEC of 25 July 1984 laying down Community methods of analysis for the official control of feedingstuffs. 11th Commission Directive 93 / 70 / EC of 28 July 1993 laying down Community methods of analysis for the official control of feedingstuffs. Twelve Commission Directive 93 / 117 / EC of 17 December 1993 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 2003 / 126 / EC of 23 December 2003 establishing an analytical method for the identification of constituents of animal origin for the official control of feedingstuffs. Commission Directive 1999 / 76 / EC of 23 July 1999 establishing a Community method of analysis for the determination of lasalocid sodium in feedingstuffs. Commission Directive 1999 / 79 / EC of 27 July 1999 amending the third Commission Directive 72 / 199 / EEC of 27 April 1972 laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 2000 / 45 / EC of 6 July 2000 laying down Community methods of analysis for the determination of vitamin A, vitamin E and tryptophan in feedingstuffs. Commission Directive 2002 / 70 / EC of 26 July 2002 laying down requirements for the determination of the levels of dioxin and dioxin-like PCBs in feedingstuffs. Commission Directive 93 / 28 / EEC of 4 June 1993 amending Annex I to Directive 72 / 199 / EEC laying down Community methods of analysis for the official control of feedingstuffs. Commission Directive 92 / 89 / EEC of 3 November 1992 amending Annex I to fourth Directive 73 / 46 / EEC laying down Community methods of analysis for the official control of feedingstuffs. '
2. In Article 1 (3), the words "pesticide residues and 'are deleted.
3. Paragraph 3 (1) reads as follows:
"(1) The total weight of all incremental samples taken from one lot of feed, feed additive or premixture is a aggregate sample; its minimum weight is set out in column 2 of Annex 3. The reduced sample is a representative part of the aggregate sample from which it is obtained by division. The final sample shall be part of a reduced sample or a homogenised aggregate sample. ';
4. In Article 3 (5), the words "with the exception of the determination of the levels of dioxins and dioxin-like polychlorinated biphenyls' shall be inserted after the words" and of prohibited substances and products in feedingstuffs'.
5. In Article 3, paragraphs 6 and 7 are added:
"(6) A aggregate sample shall be considered to represent the sampled lot or sublot.
(7) The determination of the level of dioxins as well as the level of dioxins similar to polychlorinated biphenyls is carried out in a laboratory sample. "
6. Paragraph 4 (1) and (2) reads as follows:
"(1) At least three final samples shall be taken from the aggregate or reduced sample, which shall constitute the quantity of the aggregate sample intended for testing; the minimum weight of the final sample is given in column 2 of Annex 5.
(2) The provisions of paragraph 1 shall not apply to aggregate samples when sampling for the assessment of the homogeneity of the additive in a premixture or feed batch using a premixture and for the assessment of the working accuracy of the mixing device for which the aggregate sample forms the final sample at the same time. ';
7. Paragraph 7 (1) reads as follows:
"(1) The methods of analysis laid down by the European Communities shall be used in the framework of professional supervision and testing. Where the method is not provided for in the rules, the check shall be carried out according to other methods of analysis, the principles of which are set out in the Annexes to this Decree. ';
8. Paragraph 7 (2), including footnote 5, reads:
"(2) The list and principles of methods of analysis for feed, feed additives and premixtures are set out in Annexes 9 to 14. The full procedures of the methods set out in Annexes 9 to 12 and the sampling aids referred to in Annex 1 shall be published in the Bulletin of the Central Audit and Examination Institute of the Agricultural Institute (5).
5) Article 10 of Act No. 147 / 2002 Coll., on the Central Audit and Examination Institute of Agriculture, as amended. '
9. In Paragraph 7 (3), "7 'is replaced by" 9'.
10.Paragraph 8 (2) reads as follows:
"(2) The sample shall be adjusted to avoid contamination or modification of its composition. Grinding, mixing and sifting shall be carried out as quickly as possible to minimise exposure to air and light. No mills or other apparatus which could cause the sample to heat above 40 ° C may be used for the treatment of the sample. The specially sensitive sample shall be crushed manually. In addition, it is necessary to ensure that the trace elements are not the instrument itself. '
11. Paragraph 8 (3) reads:
"(3) If the sample cannot be adjusted without substantially changing its moisture content, the moisture content shall be determined before and after treatment in accordance with the method set out in Annex 7. ';
12. In Paragraph 11 (5), the words:
"for the content in g / kg:
| pro obsah | |
| do 9,99 g/kg | s přesností na 0,01 g/kg |
| pro obsah od 10 g/kg | |
| do 99,9 g/kg | s přesností na 0,1 g/kg |
| pro obsah | |
| nad 100 g/kg | s přesností na 1 g/kg“ |
shall be replaced by:
"to express content in%:
| pro obsah do 0,999 % | s přesností na 0,001 % |
| pro obsah od 1,0 do 9,99 % | s přesností na 0,01 % |
| pro obsah nad 10,0 % | s přesností na 0,1 % |
For the expression of vitamin A content in g / kg
| do 999 m.j./kg | s přesností na 1 m.j./kg |
| od 1 000 do 9 999 m.j./kg | s přesností na 10 m.j./kg |
| od 10 000 do 99 999 m.j./kg | s přesností na 100 m.j./kg |
| od 100 000 do 999 999 m.j./kg | s přesností na 1 000 m.j./kg |
| nad 1 000 000 m.j./kg | s přesností na 10000 m.j./kg“. |
13. in Article 11 (7), the words "and 17" shall be deleted and the words "No 9, 10" shall be replaced by the words "No 9 and 10."
14. In Article 11, the following paragraph 8 is added:
"(8) The limit of determination is the value of the smallest quantity of the determined substance that can be reliably quantified. ';
15. In Annex 6, point 7 is added:
'7. The methods of sampling for the professional supervision and testing of the levels of dioxins (PCDD / Fs) and the determination of dioxin-like PCBs in certain feedingstuffs are set out in Part 1 of Annex 18. ';
16. The first paragraph of point 2 of Annex 7 reads as follows:
"If the preparation of the sample cannot be carried out without significant changes in the moisture content of the sample, the moisture content must be determined before and after the preparation of the sample according to the following test method. '
17. In Annex 7, point 3.7 is added:
"3.7. Sample preparation procedures and requirements for analytical methods used to perform expert surveillance and analysis of the levels of dioxins (PCDD / Fs) and dioxin-like polychlorinated biphenyls in certain feedingstuffs are set out in Annex No 18, Part 2 '.
18. Point 1.1 of Annex 8 reads as follows:
"1.1. Samples intended for laboratory testing of vitamin content or light sensitive substances shall be kept in packs which prevent light access. ';
19.
"Annex No 9 to Decree No. 124 / 2001 Coll.
Methods of analysis of feed, feed additives and premixtures
List of chemical methods for the analysis of feed
| 1. | Vlhkost, těkavé látky |
| 1.1. | Stanovení obsahu vlhkosti, metoda převzatá ze směrnice Komise 71/393/EHS, doplněná směrnicí Komise 73/46/EHS |
| 1.2. | Stanovení obsahu vlhkosti v olejích a tucích, metoda převzatá ze směrnice Komise 73/46/EHS |
| 2. | Dusíkaté sloučeniny |
| 2.1. | Stanovení obsahu dusíkatých látek, metoda převzatá ze směrnice Komise 93/28/EHS |
| 2.2. | Stanovení obsahu bílkovin |
| 2.3. | Stanovení obsahu aminokyselin, metoda převzatá ze směrnice Komise 98/64/ES |
| 2.4. | Stanovení obsahu močoviny, metoda převzatá ze směrnice Komise 71/250/EHS |
| 2.5. | Stanovení obsahu těkavých dusíkatých látek, metoda převzatá ze směrnice Komise 71/393/EHS |
| 2.6. | Stanovení aktivity ureázy v sóji a jejích produktech, metoda převzatá ze směrnice Komise 71/250/EHS |
| 2.7. | Ureázový test |
| 2.8. | Stanovení obsahu biuretu |
| 2.9. | Stanovení obsahu hydroxyanalogu methioninu |
| 2.10. | Stanovení obsahu dusíkatých látek rozpustných působením pepsinu, metoda převzatá ze směrnice Komise 72/199/EHS |
| 2.11. | Stanovení aktivity pepsinu, metoda převzatá ze směrnice Komise 72/199/EHS |
| 2.12. | Stanovení obsahu methioninu v premixech |
| 2.13. | Stanovení obsahu tryptofanu, metoda převzatá ze směrnice Komise 2000/45/ES |
| 3. | Tuk |
| 3.1. | Stanovení obsahu oleje a tuku, metoda převzatá ze směrnice Komise 98/64/ES |
| 3.2. | Stanovení čísla kyselosti tuku |
| 3.3. | Stanovení obsahu nerozpustných nečistot v tucích a olejích |
| 3.4. | Stanovení obsahu nezmýdelnitelných látek v tucích a olejích |
| 3.5. | Stanovení peroxidového čísla |
| 3.6. | Stanovení obsahu lecitinu |
| 3.7. | Stanovení obsahu mastných kyselin |
| 4. | Polysacharidy |
| 4.1. | Stanovení obsahu vlákniny, metoda převzatá ze směrnice Komise 92/89/ES |
| 5. | Bezdusíkaté látky výtažkové |
| 5.1. | Stanovení obsahu škrobu, metoda převzatá ze směrnice Komise 99/79/ES |
| 5.2. | Stanovení obsahu cukrů, metoda převzatá ze směrnice Komise 71/250/EHS |
| 5.3. | Stanovení obsahu laktosy, metoda převzatá ze směrnice Komise 71/250/EHS |
| 5.4. | Stanovení obsahu bezdusíkatých látek výtažkových výpočtem |
| 6. | Popel |
| 6.1. | Stanovení obsahu popele, metoda převzatá ze směrnice Komise 71/250/EHS |
| 6.2. | Stanovení obsahu popele nerozpustného v kyselině chlorovodíkové, metoda převzatá ze směrnice Komise 71/250/EHS |
| 7. | Makroprvky |
| 7.1. | Stanovení obsahu fosforu, metoda převzatá ze směrnice Komise 71/393/EHS |
| 7.2. | Stanovení obsahu hořčíku, metoda převzatá ze směrnice Komise 73/46/EHS |
| 7.3. | Stanovení obsahu draslíku, metoda převzatá ze směrnice Komise 71/250/EHS |
| 7.4. | Stanovení obsahu sodíku, metoda převzatá ze směrnice Komise 71/250/EHS |
| 7.5. | Stanovení obsahu ve vodě rozpustných chloridů, metoda převzatá ze směrnice Komise 71/250/EHS |
| 7.6. | Stanovení obsahu celkových uhličitanů, metoda převzatá ze směrnice Komise 71/250/EHS |
| 7.7. | Stanovení celkového obsahu síry |
| 7.8. | Stanovení obsahu vápníku, metoda převzatá ze směrnice Komise 71/250/EHS |
| 8. | Mikroprvky |
| 8.1. | Stanovení obsahu mědi, železa, manganu a zinku, metoda převzatá ze směrnice Komise 78/633/EHS |
| 9. | Kyselost |
| 9.1. | Stanovení volné, vázané a celkové kyselosti vodního výluhu |
| 9.2. | Stanovení kyselosti vodního výluhu v mléčných krmných směsích |
| 10. | Nežádoucí látky |
| 10.1. | Stanovení obsahu kyanovodíku, metoda převzatá ze směrnice Komise 71/250/EHS |
| 10.2. | Stanovení obsahu glukosinolátů v řepce |
| 10.3. | Stanovení obsahu 5-vinyl-2-thiooxazolidonu |
| 10.4. | Stanovení obsahu kyseliny erukové |
| 10.5. | Stanovení obsahu olova a kadmia |
| 10.6. | Stanovení obsahu ricinových slupek |
| 10.7. | Stanovení obsahu reziduí organochlorových pesticidů |
| 10.8. | Stanovení obsahu indikačních kongenerů polychlorovaných bifenylů (PCB) |
| 10.9. | Stanovení obsahu toxaphenu |
| 10.10. | Stanovení obsahu rtuti |
| 10.11. | Stanovení obsahu aflatoxinu B1, metoda převzatá ze směrnice Komise 76/372/EHS, doplněná směrnicí Komise 92/95/EHS a 94/14/ES |
| 10.12. | Stanovení obsahu arsenu |
| 10.13. | Stanovení obsahu gossypolu, metoda převzatá ze směrnice Komise 72/199/EHS |
| 10.14. | Stanovení obsahu theobrominu |
| 11. | Zkoušení siláží |
| 11.1. | Zkoušení jakosti siláží |
1. Humidity, volatile substances
1.1. Determination of moisture content
Purpose, scope and principle
The method makes it possible to determine the moisture content of feedingstuffs.
The method is not suitable for determining the moisture content of milk products for direct feeding, for minerals and mixtures which are mainly composed of mineral substances, for animal and vegetable fats and oils or for analysis of oil seeds and fruits.
Samples shall be dried under prescribed conditions depending on the origin of the feed. The moisture content is determined by weight as weight loss. For solid feedingstuffs with a high moisture content, pre-drying is required.
Repeatability
The difference between two parallel determinations carried out on the same sample shall not exceed 0,2%.
Reproducibility
Not fixed.
1.2. Determination of moisture content of oils and fats
Purpose, scope and principle
The method allows the determination of water and volatile substances in animal and vegetable fats and oils.
The sample is dried to constant weight at 103 ° C. Weight loss is determined by weighing.
Repeatability
The difference between two parallel determinations carried out on the same sample shall not exceed 0,05%.
Reproducibility
Not fixed.
2. Nitrogen compounds
2.1. Determination of nitrogen content
Purpose, scope and principle
The method specifies the conditions for determining the nitrogen content of feed based on the determination of the nitrogen content by the Kjeldahl method.
The sample is mineralized with hot sulphuric acid in the presence of a catalyst. The acid solution is alkali with sodium hydroxide solution. The ammonia is distilled and added to the measured amount of sulphuric acid and the excess is titrated with a graduated sodium hydroxide solution.
Repeatability
The difference between two parallel determinations carried out on the same sample shall not exceed:
| do 20 % | 0,2 % |
| od 20 do 40 % | 1 % relat. |
| nad 40 % | 0,4 % |
Reproducibility
Not fixed.
2.2. Determination of protein content
Purpose, scope and principle
The method specifies the conditions for determining the protein content of feed and is applicable to all feed of organic origin.
The protein content shall be determined by the Barnstein method after their separation from nitrogen matter of non-white origin by precipitating copper salts.
Repeatability
Not fixed.
Reproducibility
Not fixed.
2.3. Determination of amino acid content
Purpose, scope and principle
This method is used to determine the content of free (synthetic and natural) and all (bound by peptidic binding and free) amino acids in feedingstuffs using an amino acid analyser. The method is suitable for the following amino acids: cyst (e) in, methionine, lysine, threonine, alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine and valine.
This method does not distinguish between amino acid salts and cannot distinguish between forms D and L. It is not suitable for determination of tryptophan or hydroxyanalogues of amino acids.
Free amino acids
Free amino acids are extracted with dilute hydrochloric acid. At the same time, the extracted nitrogenous macromolecular substances are precipitated by sulphosalicylic acid and removed by filtration. Adjust the filtered solution to a pH of 2,20. The amino acids are separated by ion-exchange chromatography and determined by photometric detection at 570 nm after reaction with ninhydrin.
All amino acids
The chosen procedure depends on the determined amino acids. Cyst (e) in and methionine must be oxidised to cysteic acid and methionine sulfone before hydrolysis. Tyrosine must be determined in non-oxidized hydrolysates. The other amino acids referred to in point 1 may be determined either in an oxidised or non-oxidised sample.
Oxidation is performed at 0 ° C by a mixture of performic acid and phenol. The excess oxidising agent is distributed with sodium disulphite. The oxidised or non-oxidised sample is hydrolysed with hydrochloric acid (c = 6 mol / l) for 23 hours. Adjust the hydrolysate to a pH of 2,20. The amino acids are separated by ion-exchange chromatography and determined by photometric detection at 570 nm (440 nm for proline) after reaction with ninhydrin.
Repeatability
Repeatability values for different amino acids and different feedingstuffs are part of the complete version of the method.
Reproducibility
Reproducibility values for different amino acids and different feedingstuffs are part of the complete wording of the method.
2.4. Determination of urea content
Purpose, scope and principle
The method specifies the conditions for determining the urea content of feed with additive content of that substance.
The urea content shall be determined, after the water leaching of the sample by Carres reagents, by reaction with p-dimethylaminobenzaldehyde spectrophotometric at a wavelength of 420 nm.
Repeatability
Not fixed.
Reproducibility
Not fixed.
2.5. Determination of volatile nitrogen content
Purpose, scope and principle
A. Microdiffusion
Purpose, scope and principle
The method specifies the conditions for determining the content of volatile nitrogen substances expressed as ammonia in feed.
The sample is extracted with water, the solution is elicited and filtered. Volatile nitrogen bases are collected in a boric acid solution and titrated with sulphuric acid after addition of potassium carbonate solution and microdiffusion.
B. Distillation
Purpose, scope and principle
The method allows the determination of the volatile nitrogen content, expressed as ammonia, in fish meal which contains virtually no urea. The method is only applicable to contents of less than 0,25% of ammonia.
The sample is extracted with water, the solution is elicited and filtered. Volatile nitrogen bases are extracted by boiling after addition of magnesium oxide and collected in the prescribed amount of sulphuric acid. The excess sulphuric acid is titrated with sodium hydroxide solution.
Repeatability
Microdiffusion
The difference between two parallel determinations carried out on the same sample shall not exceed:
| do 1,0 % | 10,0% relat. |
| nad 1,0 % | 0,1 % |
Distillation
The difference between two parallel determinations carried out on the same sample shall not exceed 10,0% of relays for all volatile nitrogen content.
Reproducibility
Not fixed.
2.6. Determination of urease activity in soy and its products
Purpose, scope and principle
The procedure specifies the conditions for determining the urease activity in soya and its products to verify that these products have been heat-treated for a sufficient period of time. The urease activity is determined by determining the amount of ammonia nitrogen released from the urea solution by one gram of test sample per minute at 30 ° C.
Repeatability
Not fixed.
Reproducibility
Not fixed.
2.7. Urease test
Purpose, scope and principle
The method specifies the conditions for determining the urease activity in soya and its products.
The urease activity shall be determined by titrating alkalimetrically the amount of ammonia released from the urea solution by urease from the sample tested per 1 hour.
Repeatability
Not fixed.
Reproducibility
Not fixed.
2.8. Determination of biuret content
Purpose, scope and principle
The method specifies the conditions for determining the biurete content of urea.
The method is based on the formation of a colour biuret complex with copper sulphate and measurement of the absorbance of the coloured solution at the wavelength 540 nm.
Repeatability
The difference between two parallel determinations carried out on the same sample shall not exceed 0,03%.
Reproducibility
Not fixed.
2.9. Determination of the hydroxyanalogue content of methionine
Purpose, scope and principle
The hydroxyanalogue content of methionine is determined after extraction of the sample with water-acetonitrile and subsequent hydrolysis by the HPLC reverse phase using UV detection.
Repeatability
Not fixed.
Reproducibility
Not fixed.
2.10. Determination of the nitrogen content of pepsin-soluble substances
Purpose, scope and principle
The method is applicable for determining the proportion of nitrogenous substances soluble in pepsin and hydrochloric acid under defined conditions.
The sample of feed in the diluted hydrochloric acid and pepsin solution is heated for 48 hours to 40 ° C. The suspension is filtered and the nitrogen content is determined in the filter using the 2.1 method.
Repeatability
The difference between two parallel determinations carried out on the same sample shall not exceed the content of the nitrogen-soluble pepsin:
| do 20 % | 0,4 % |
| od 20 do 40 % | 2 % relat. |
| nad 40 % | 0,8 % |
Reproducibility
Not fixed.
2.11. Determination of Pepsin activity
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Regulation Information
| Citation | Decree No. 497 / 2004 Coll., amending Decree No. 124 / 2001 Coll., laying down the requirements for sampling and the principles of methods of analysis of feed, additives and premixtures and the method of storage of samples |
|---|---|
| Regulation Type | Order |
| Author | - |
| Collection | Code of Laws |
| Date of Promulgation | 22.09.2004 |
|---|---|
| Effective from | 01.10.2004 |
| Effective until | - |
| Status | Valid |
Legal Areas:
Administrative law
Agriculture
The regulation text is for informational purposes only.
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