Decree No. 156 / 2021 Coll.
Decree amending Decree No. 61 / 2011 Coll., on the requirements for sampling, procedures and methods for testing seed and seed, as amended
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156
DECLARATION
of 30 March 2021
amending Decree No 61 / 2011 Coll., on the sampling requirements, procedures and methods for testing seed and seed, as amended
The Ministry of Agriculture shall determine, pursuant to Articles 3a (11), 4 (15) (a) and (b), 5 (8) (h), 6 (8) (c) to (f), 19 (17) (l) and 22 (10) (b) of Act No. 219 / 2003 Coll., on the putting into circulation of seeds and propagating plants and on the amendment of certain laws (Act No. 281 / 2009 Coll., Act No. 331 / 2010 Coll., Act No. 54 / 2012 Coll., Act No. 279 / 2013 Coll., Act No. 243 / 2016 Coll., Act No. 183 / 2017 Coll., Act No. 295 / 2017 Coll.
Decree No. 61 / 2011 Coll., on the requirements for sampling, procedures and methods of testing seed and seed, as amended by Decree No. 410 / 2013 Coll. and Decree No. 3 / 2018 Coll., is amended as follows:
1. Paragraph 1 (2) reads as follows:
"(2) For the purposes of this decree:
(a) a partial sample of a lot of seed or seed taken from a lot of seed or seed in a single sampling operation;
(b) by an aggregate sample, a sample resulting from the sprinkling and mixing of all incremental samples taken from one lot;
(c) a laboratory sample of the prescribed minimum mass referred to in Annex 5 intended to be sent to the test laboratory, consisting either of the whole aggregate sample or of a division of the aggregate sample,
(d) by a reserve sample, a sample intended for storage for any further tests prepared from the same aggregate sample and in the same way as the laboratory sample; each reserve sample must bear the designation "Reserve sample 'with the symbol" R',
(e) a revision sample for re-assessment of the health status of seed potatoes,
(f) adjustment operations involving the packaging, closure and labelling of a sample or seed lot;
(g) by the sampled staff member of the Central Audit and Examination Institute of the Agricultural Institute (hereinafter referred to as the Institute) or a natural person authorised by the Institute for Sampling pursuant to Article 17 (3) of the Act,
(h) by an official sample, a sample taken and supplemented by a sample;
(i) a sample for the growing test, a sample intended for the growing test for the determination of species and varietal purity and authenticity, health status or hybrid;
(j) a common sample taken together and adjusted by the supplier and the buyer;
(k) a private sample sample which does not comply with the requirements of the official or common sample;
(l) a standard sample of a variety intended for comparison in plant and laboratory tests for varietal identity,
(m) a sample taken by a sample sampled by a sampled official who is an employee of the Institute for the purpose of checking the quality of propagating material put into circulation or for the purposes of official supervision pursuant to Article 17 (14) of the Act;
(n) a sample of the weight of the test sample set out in Annex 5 and intended for the determination of the mixtures of other plant species in pieces;
(o) a basic test sample weighing as set out in Annex 5 and intended to determine the purity of the seed as a percentage,
(p) the homogeneity of seed lots within natural variability;
(q) heterogeneous seed inconsistency of a seed lot in which the individual components of the quality characterisation are not evenly distributed,
(r) a sample for the determination of the health status of seed potatoes, the number of tubers collected after completion of vegetation referred to in Annex 1;
(s) an official or reserve sample packaging;
(t) sampling for sampling;
(u) an employee of the Institute or a natural person appointed by the Institute to carry out inspections of seed crops pursuant to Article 17 (2) of the Act. "
2. Under Section 2, "Paragraph 19 (15) 'is replaced by" Paragraph 19 (17)'.
3. In Article 2 (b), the words "and the estimate of the harvest 'are deleted.
4. In Article 2, at the end of the text of point (d), the words "the registration of used labels may be submitted electronically 'shall be inserted.
5. in Article 2 (f):
"(f) in the case of seed potatoes completed with the label of the sample for determining the health status of seed potatoes, the model of which is published on the Institute's website."
6. In the text under the group title above § 3, the text "§ 6 (10) 'is replaced by" § 6 (8)'.
7. In Article 3 (1), the words "sampling (sampling) 'are replaced by the words" sampling'.
8. In Article 3, at the end of paragraph 2, the sentence "Sampling for the purpose of issuing international certificates is only allowed to be carried out by a sample collector who is an employee of the Institute."
9. In Paragraph 3 (4), "12 'is replaced by" 14'.
10. in Article 4 (1), the words "in the mixture of the highest weight percentage" shall be replaced by the words "the maximum weight of the laboratory sample."
11. in Article 5 (6) and (7):
"(6) The minimum sizes of seed samples and seed potatoes samples for testing the varietal identity and varietal purity are set out in Annex 5. The model of the application for electrophoresis is published on the Institute's website.
(7) 3 samples shall be taken to check the ingredient of the genetically modified variety in the seed, one of which shall be intended for testing in the laboratory, the other kept by the supplier as a reserve sample and the third shall be stored at the Institute for the event of repeated testing. Samples shall be stored in such a way that they cannot be degraded. The minimum sample sizes for the detection of the presence of genetic modifications in seeds are set out in Annex 5. ';
12. in Paragraph 7 (1), the last word "sample maker" shall be replaced by "supplier."
13. in Article 9 (2), the words "seed potatoes" shall be inserted after the word "sample," and the words "Annex No 2" shall be replaced by "published on the Institute's website."
14. in Article 9 (3), the words "set out in Annex No 2" are replaced by the words "are published on the Institute's website."
15. In Section 10, at the end of paragraph 5, the sentence "The laboratory responsible may dispose of the sample early only on authorisation of emergency disposal issued by the Institute. 'is added.
16. In the text under the group heading above § 11, the words "[K § 5 (9) (h), § 6 (10) (f) and (e) and § 22 (10) (b) of the Act 'are replaced by" [K § 5 (8) (h), § 6 (8) (e) and (f) and § 22 (10) (b) of the Act'.
17. in Paragraph 11 (4), the words "and the model label of the sample for the growing test are given in Annex 2" shall be replaced by the words "published on the website of the Constitution."
18. In Article 12 (1), the words "the presence of warehouses' shall be inserted after the words" and samples to be determined '.
19. in Paragraph 12 (2), the second sentence is deleted;
20. Paragraph 12 (3) reads:
"(3) The seed sampling requirements for the purpose of detecting the presence of genetic modifications are set out in Section 5 (7) and Annex 5. The procedures and methods for detecting the occurrence of genetic modifications in seed and seed samples are set out and published on the Institute's website. ';
21. Paragraph 13 (2) reads as follows:
"(2) The experiments and tests referred to in paragraph 1 are intended to check compliance with conditions to be met by seed and seed put into circulation and to harmonise the methods used for certification of seed and seed."
22. Annexes 1 to 5 shall read:
"Annex No 1 to Decree No 61 / 2011 Coll.
Techniques and methods of sampling of seed and seed seed and seed lot heterogeneity
1. Technical aids
(1) sampling tool or device (point sampling device, two-shell sampling device, automatic sampling device),
(2) a child,
(3) bucket and blade for mixing the sample,
(4) scales,
(5) stitching needle, twine,
(6) sample panel (multilayer paper bag),
(7) airtight packaging (e.g. PE or PP bottle) for the collection of seed for the determination of moisture and, where appropriate, for the detection of the presence of pests in storage,
(8) cloth or other breathable bag,
(9) the working surface (table) for the adjugation of the sample and administrative activity during sampling.
(10) new breathable packaging for sampling seed potatoes.
2. Subsamples taken
(1) The number or size of the incremental samples taken shall be chosen by the sampled operator in such a way that the minimum sampling intensity required in Part 3 is met, while at the same time sending to the laboratory the minimum quantity of seed required for the required tests and the creation of a reserve sample.
(2) During sampling, individual incremental samples of approximately the same size shall be taken, regardless of the lot or packaging location the samples are taken from.
(3) Where seed lots are in packages, the packaging shall be selected for sampling from the whole lot at random or according to a predetermined plan. Partial samples shall be taken from the top, middle and bottom of the packaging but not necessarily from more than one site of one packaging, unless this is necessary to meet the minimum sampling intensity specified in Part 3.
(4) Where seed lots are in bulk or in bulk, partial samples shall be taken from randomly selected places.
(5) The packaging shall be opened or pierced when the incremental samples are taken. The packaging from which the partial sample has been taken shall then be closed or its contents transferred to new packaging.
(6) Where seed lots are to be packed in specific types of packaging (for example, small packages, moist packaging, impermeable packaging), partial samples shall be taken, if possible, before or during packing.
(7) When sampling seed lots in the form of seed bands or mats, the whole packages or parts of strips or mats shall be collected.
(8) The instruments used for sampling shall not damage or select the seeds on the basis of their size, shape, bulky weight, oainess or any other characteristic. All sampling tools shall be clean before sampling to avoid possible contamination. The samples shall be long enough to reach at least the centre of the container near the pointy end. If the packaging is not accessible from both opposite sides, the sampling device shall be long enough to reach the opposite side of the packaging. When sampling seed lots, one of the following methods shall be used.
2.1. Methods of sampling
2.1.1. Point (Nobbe) Sample
(1) It consists of a tube with an opening at the pointed end. The seed passes through the tube and is collected into a suitable container. The minimum inside diameter of the Nobbe sampling device shall be sufficiently wide to allow for free and smooth flow of seeds and impurities by sampling.
(2) In the case of use, the sampling device with an opening facing downwards shall be inserted into the seed at an angle of about 30 ° relative to the horizontal plane. The sample inserted into the required depth shall be rotated around the longitudinal axis by 180 ° and then removed from the packaging at a constantly decreasing rate, shaking gently to maintain a uniform flow of seed. The seed is collected in a suitable container.
2.1.2. Two-casing sampling device
(1) It consists of an inner tube which fits freely into the inside of the outer tube, but which is so tight that neither seeds nor impurities can slip into the gap between them. The outer tube has a firm pointed end. The holes are carved into the walls of both pipes, so that the inner tube cavity can be opened and closed by turning both pipes against each other. The recommended dimensions of the sampling device are: the length of the sampling device such that the sampling device reaches the opposite wall of the packaging; in the case of vertical sampling of packaging, the two-shell sampling device must reach the bottom of the packaging; the width of the hole is at least twice the maximum diameter of the seeds or impurities that may be present in the lot; the length of the opening is at least twice the width of the hole; the maximum length of the opening may be as long as the whole sampling unit. The minimum inside diameter of the double-shell sampling device shall be sufficiently wide to allow free and smooth flow of seeds and impurities into the sampling device.
(2) The two-shell sampling device can be used in a horizontal, vertical or oblique position. A two-shell sampling device with holes arranged in spirals, which are opened gradually from tip to handle, may only be used for seeds smaller than the seed of the species Triticum aestivum.
(3) If a two-shell sampling device is used vertically or in an oblique position, it shall have either compartments dividing the inner space of the sampling device into several chambers or holes arranged in a spiral.
(4) The sample shall be inserted into the seed in a closed state, the tube shall rotate each other so that the cracks of the inner tube are opened and shaken slightly to fill with the seed. It is then closed again, withdrawn and emptied into a suitable hopper or on a suitable pad. The closure must be carried out in such a way as not to damage the seed. A single incremental sample shall be considered as the whole content of such a type of sampling device, not the content of one of its chambers.
2.1.3. Sample for large costs and bulk seed
(1) It consists of a separate chamber attached to the handle. The lower part of the chamber is a conical shape with a pointed end. To achieve greater depth, the handle can be gradually extended by screwing other attachments. In the chamber there is a closing system which can be formed either by a ring on the outside of the tool or by a wing connected with a door or a valve pressed by a spring. Some of these samples can be closed before being pulled from the sampling site, others cannot be closed, so the filled chamber is pulled out in an open state. For all species, the minimum inside diameter of the chamber may be about 35 mm and 75 mm high.
(2) The sample shall be placed in the seed in a closed state, gently pressed so that the tip reaches the desired position, pulled back approximately 10 cm, or rotated (according to the sealing system), shaken slightly to fully fill with the seed, carefully closed, if possible, pulled out and emptied into a suitable container. The closure must be carried out in such a way as not to damage the seed.
2.1.4. hand sampling
(1) The sampling method is most appropriate for the following genera: Agryron, Agrostis, Alopecurus, Anthony anthus, Arrhenatherum, Bromus, Cynthia, Cynosurus, Dactylis, Descampsia, Elytrigia, Festuca, Holcus, Lolium, Panicum, Poa and Trisetum. This method is also most appropriate for the sampling of seeds which could be damaged by the use of samples such as large seed legumes, seeds with wings or seeds with low moisture, as well as for seed belts and seed mats.
(2) For the collection of incremental samples of hands from seed in packages, all places within packages shall be accessible. For this purpose, the packaging may be partially or completely emptied during sampling. Packs in which all layers of seed are not accessible by normal opening may need to be cut, sampled and then re-packed.
(3) Hand sampling shall be carried out by inserting a clean open hand into the required depth of the seed, clamping the palm and pulling the hand out, making sure that the fingers around the captured seed are firmly clamped so that no seed can escape from the palm.
2.1.5. Sampling using an automatic sampling device
2.1.5.1. Use of automatic sampling device
(1) Partial samples shall be taken from the seed stream when filling into packaging.
(2) Requirements for the operation of an automatic sampling device
(a) the incremental samples shall be taken from the seed stream evenly throughout its cross-section and the frequency of their collection shall be controlled by a time switch, the intervals between the incremental samples being uniform;
(b) the incremental samples are carried by a line from the sampling device to the container in the collection cabinet;
(c) the seeds of this establishment must not fall, by reflection or otherwise, outside the collection vessels;
(d) the contents of one vessel must correspond to the size of the aggregate sample;
(e) the collection cabinet shall be lockable;
(f) the sampling cabinet, the time switches and all seed management connections from the sampling unit to the collection cabinet shall be sealed;
(g) the sampling agent must not damage or select the seed between the seeds on the basis of their size, hoverness or plurality.
(3) The automatic sampling device shall be installed according to the manufacturer's technological conditions and its operation for the purpose of official sampling shall be approved by the Institute.
(4) When using an automatic sampling device for the purpose of official sampling, this sampling device shall be locked and accessible only to the sampling operator.
(5) Additional requirements for the operation and security of an automatic sampling device
(a) the sampling mechanism, including the time switch, shall be placed in a lockable housing;
(b) the piping between the sampling device and the collection cabinet shall be so long that the fall of seed into the collection vessel cannot cause any damage to the seed sampled;
(c) if the manifold is divided and duplicated above the collection cabinet, a sample shall be collected for the laboratory of the cleaning station into a container outside the collection cabinet;
(d) the collection cabinet is firmly attached to the ground and secured against any displacement, opening by itself and shall comply with the following conditions:
1. there shall be no holes in the shell other than a hole for the seed supply, a slot for the insert of the ticket indicating the lot number, an opening for the outlet of the external control mechanism of the rotating device with collecting vessels and a sufficiently spacious handling hole for the removal of sample collection vessels;
2. inside it contains a rotary device with individual collecting containers. The control of this device shall be so designed that it is possible to operate the fifth wheel in only one direction within 360 ° outside the case without the possibility of reversing; the reverse movement of the fifth wheel, or turning to the starting position, is only possible when the cabinet is open,
3. the individual containers must be evenly distributed so that, even if more seed is present, the sample cannot be degraded by mixing seed from several containers,
4. the key to the cabinet has a sample maker, the second key is stored in the sample-sealed envelope at the responsible cleaner's office,
(e) the time intervals of the switch shall be set so that, in the time needed to clean the seed lot, so many incremental samples are taken by sampling, corresponding to approximately four times the laboratory sample by weight;
(f) the sampling by automatic sampling shall take place throughout the cleaning process of the sampled lot.
(6) If the time pulse setting does not correspond to the capacity of the collection vessel, two collection vessels with the prescribed marking may be used for a sample from one batch.
(7) The operation of the automatic sampling device shall be recorded in the book of the automatic sampling device with data identical to those on the leaves thrown into the collection vessels supplemented by the type, variety and weight of the sampled lot. This register shall be checked by the staff member of the Institute.
2.1.5.2. Procedure for operating an automatic sampling device
(1) Before cleaning the seed lot, the operator shall throw a ticket with the lot number, date and hour of start of cleaning the lot into the collection container with a slot and record in the book of the automatic sampling device.
(2) Upon completion of the cleaning of the lot, the operator will again throw a ticket with the lot number, the date and hour of completion of the cleaning of the lot and record in the book of the automatic sampling device.
(3) They turn the turntable one place so that under the seed supply there is an empty container, and throw the ticket for the next lot.
(4) The procedure shall be repeated as many times as the number of lots is prepared between the individual official sampling of samples by the sampling agent or if the capacity of the collection cabinet is sufficient.
2.1.5.3. Sampling procedure
(1) The sample shall take aggregate samples from the automatic sampling cabinet and prepare a laboratory, reserve and, where appropriate, other official sample, checking the conformity of the data on both leaves placed in the container with the data in the records submitted.
(2) After all the samples have been taken, the collecting vessels shall be placed back on a rotary device which shall be adjusted to the starting position and the collection cabinet shall be closed and locked.
(3) If the sample in the collection container does not have the necessary mass, the whole new sampling shall be carried out by hand.
2.1.5.4. Approval and control of an automatic sampling device
(1) The replacement, repair of a larger scale or installation of a new automatic sampling device and its entry into service, in the sense of sampling for certification procedures, shall be subject to approval by the Institute. The approval process shall check the above requirements for setting, securing and correct operation of the automatic sampling device. Part of the approval process is the confluence of manual sampling and sampling by an automatic sampling device from each lot and comparison of laboratory values of these samples in the Institute's laboratory against each other. The sample for the recognition procedure shall in this case be a manual sample, the sample taken by an automatic sample shall be a control sample. The number of lots so collected shall be determined by the Institute from the course of the results.
(2) The accuracy of sampling and the reliability of the work of each automatic sampling device are checked at least twice at the beginning of the season by means of a sample from the same batch taken by the manual sampling operator who is an employee of the Institute, once during the sampling of spring crop types and once during the sampling of winter crop types. The sample taken by automatic sampling and the sample taken manually shall be compared in the laboratory of the Institute against each other. The sample for the recognition procedure shall in this case be the sample taken by an automatic sampling device and the manual sampling shall be the control sample. If the laboratory values of the two samples show a difference in tolerance, the Institute shall order more frequent checks and, where appropriate, undoing of the automatic sampling device.
2.1.6. Manual sampling of seed stream
Partial samples may be taken from the seed stream using hand tools when filling the packaging, provided that the incremental samples are taken from the seed stream evenly throughout its cross-section and that the seeds from the instrument are not reflected or otherwise look spontaneously.
3. Number of incremental samples in relation to the type and number of packages
(1
(a) For packages containing 15 to 100 kg of seed, the minimum number of incremental samples referred to in Table 1 shall be:
Table 1 - Minimum number of incremental samples taken for seed lots in packages containing up to and including 100 kg of seed
| Počet obalů v partii | Minimální počet dílčích vzorků |
|---|---|
| 1 | 5 dílčích vzorků |
| 2 - 4 | 3 dílčí vzorky z každého obalu |
| 5 - 8 | 2 dílčí vzorky z každého obalu |
| 9 -15 | 1 dílčí vzorek z každého obalu |
| 16 - 30 | 15 dílčích vzorků, každý z jiného obalu |
| 31 - 59 | 20 dílčích vzorků, každý z jiného obalu |
| 60 - 154 | 30 dílčích vzorků, každý z jiného obalu |
| 155 - 400 | 1 dílčí vzorek z každých 5 obalů, každý vzorek z jiného obalu |
| 401 - 566 | 80 dílčích vzorků, každý z jiného obalu |
| 567 a více | 1 dílčí vzorek z každých 7 obalů, každý vzorek z jiného obalu |
(b) In the case of packages containing less than 15 kg of seed, the packaging shall be merged into sampling units not exceeding 100 kg, such as 20 packages of 5 kg, 33 packages of 3 kg or 100 packages of 1 kg. The sampling units shall then be considered as packaging and sampling shall take place in accordance with Table 1.
(c) In the case of seed in pellets, granules, seed bands and seed mats, packages containing less than 300 000 seeds shall be merged into sampling units not exceeding 2 000 000 seeds. The sampling units shall then be considered as packaging and sampling shall take place in accordance with Table 1.
(2) For the sampling of seed in packages containing more than 100 kg of seed (including bulk seed) or from a stream of seed during filling of packages, the minimum number of incremental samples taken as shown in Table 2 shall be respected:
Table 2 - Minimum number of incremental samples taken for seed lots in packages containing more than 100 kg of seed or from a seed stream
| Hmotnost partie | Minimální počet dílčích vzorků |
|---|---|
| do 500 kg | nejméně 5 dílčích vzorků |
| 501 až 3 000 kg | jeden dílčí vzorek z každých 300 kg, nejméně však 5 dílčích vzorků |
| 3 001 až 20 000 kg | jeden dílčí vzorek z každých 500 kg, nejméně však 10 dílčích vzorků |
| 20 001 a více kg | jeden dílčí vzorek z každých 700 kg, nejméně však 40 dílčích vzorků |
(3) When sampling a lot with a number of packages of 15 or less, the same number of incremental samples shall be taken from each package regardless of their size. The minimum number of incremental samples to be taken for packaged seed (seed pelleted, incubated, granulated, seed strip and mats) shall be given in Tables 1 and 2.
4. Sampling to determine warehouse pests
(1) For the determination of storage pests, the incremental samples shall be taken directly into an airtight container and the aggregate sample thus obtained shall be sent directly for analysis. Samples shall be taken alternately
(a) from the bottom of the packaging (up to 200 mm high),
(b) from a part of a packaging exceeding 200 mm from the circumference layer,
(c) from a part of the packaging above 200 mm from the inner layer.
(2) The number of incremental samples is set out in Table 1.
(3) Semi-samples shall be taken from seed in bulk packaging and bulk seed in places where storage pests can be expected to occur (cloudy places, places near the floor, walls, etc.).
(4) The size of samples for the detection of pests in storage is the same as the prescribed weight for the laboratory sample, except for oil and fibre plants for which 250 g is prescribed.
5. Preparation of the aggregate and laboratory sample
(1) The aggregate sample is obtained by merging the incremental samples if it appears to be homogeneous. If the incremental samples do not appear to be homogeneous, sampling of the lot shall be stopped. If the incremental samples are collected directly into a single container, the contents of the container may be considered as a aggregate sample only if the seed in the container appears to be homogeneous. Otherwise, the contents of the container cannot be used to obtain a laboratory sample. When mixing the aggregate sample by repeated use of one of the mechanical dividers, the sample shall not be mixed manually between each use of the angel.
(2) If it is difficult to mix and reduce the aggregate sample under storage conditions, it is delivered to the laboratory for reduction. The aggregate sample shall correspond in size to the number of samples prepared. The sample shall be taken for the moisture test in an airtight container and the aggregate sample sealed. Together with a sample for the moisture test and samples marked by the supplier, it shall send the aggregate sample to the laboratory of the Institute.
(3) The laboratory sample, the reserve sample and, where appropriate, any other sample at the supplier's request is prepared by mixing and reducing the aggregate sample to an appropriate size by one of the following methods, in the case of a large aggregate sample, one of the incremental sampling methods may be used.
(a) Mechanical separation - is suitable for all seed species, except for some very difficult-to-sprinkle species, which are the species of Anthony Anthanthus, Arrhenatherum and Trisetum. Different types of mechanical dividers shall be carried out which shall meet the following requirements:
1. to provide at least one representative part of the total quantity of the sample divided,
2. to divide the heterogeneous material or mixture of seeds of different sizes and different physical characteristics of seeds and additives correctly,
3. there shall be no change in the composition of the cut-off material and the function of the dilly during division (electrostatic charge, capture and additional release of certain components),
4. maintain a steady working speed of moving parts exceeding 1 m / s and the diameter of the paths through which the material passes shall be at least 2,5 times the maximum seed size;
5. maintain a constant dividing ratio with a tolerance of ± 3%.
b) Method of dividing hands - its use is limited to the families Anthony Antoxanthus, Arrhenatherum and Trisetum and the families of fragile seeds susceptible to damage which are the families Arachis, Glycine and Phaseolus. For all other species it may only be used to obtain test samples in the health testing laboratory. The seed is spread evenly onto a smooth clean surface, mixed thoroughly with a straight edge with a spatula and formed into a pile which then is divided in half, three times in a row in total, so that eight parts are formed. These will be arranged in two rows of four and then mixed all odd parts together and all even parts together, creating two halves of the original seed quantity.
(c) Method of dividing by spoon - recommended for the preparation of a sample for health testing, for other tests limited to species with seeds smaller than wheat seeds. After pre-mixing, the seed shall be spread evenly onto a tray which is then no longer shaken, and the seed shall be collected from at least five randomly selected places using a spoon and spatula.
d) Modified halving method - the tool consists of a tray and a grid which missing the bottom of half the cubic cells. After preliminary mixing, the seed shall be spread evenly onto the grid. After lifting the grid, approximately half of the sample will remain on the tray.
(4) After one test sample or half test sample has been obtained, the remainder of the laboratory sample shall be re-mixed before preparation of the next test sample or half test sample.
(5) When preparing the sample for the moisture test, it shall be carried out to minimise changes in the moisture content of the sample. The samples shall be taken from the aggregate sample by mixing or splitting the aggregate sample through the mechanical divide and re-mixing, no more than three times, then taking a sample of the required size from three different locations in the immediate packaging, including the upper, middle and lower parts of the aggregate sample.
(6) In the case of seed bands and seed mats, parts of the belts or mats shall be taken at random.
(7) Where one of the said divisions is used to prepare a sample of pelleted seed, the drop height shall not exceed 250 mm.
6. Sampling of seed potatoes
(1) Sampling for health determination
Sampling of seed potatoes for the purpose of determining disease infestations shall be carried out either directly from seed crops or from warehouses. The samples shall be taken from the warehouses by the samples of control, revision and, where appropriate, from lots of seed for which no sampling from the crop has been carried out.
(a) Procedure for sampling seed potatoes from seed crops:
1. the aggregate sample shall be obtained by merging five incremental samples, which shall be taken from five different positions of the chessboard spread over the plot, each from two adjacent lines, in each of the 11 consecutive stacks,
2. one or two seed size tubers are collected from each rind. When two tubers are taken, one tuber shall be placed in the laboratory sample and the other in the field growing test sample,
3. One sample contains 110 tubers.
(b) Where a sample has not been taken from the crop, tubers of an average size shall be taken at random from stored seed, one sample containing 110 tubers.
(2) Sampling of seed for mechanical analysis
(a) partial samples of seed potatoes after sorting shall be taken at random by holding both hands, including gross impurities, so that the sample represents the average seed condition;
(b) where the analysis of the sample is not carried out on the spot, it shall be sent for analysis in packages which must be dry, clean and well breathable and marked in accordance with the model of the sample label for mechanical analysis of seed potatoes published on the Institute's website. The packaging is provided by the supplier.
7. Sampling of onion and garlic seed
(1) Each incremental sample shall be taken alternately from the top, middle and bottom lots of onion and garlic in packages weighing more than 30 kg in the quantity needed to complete the aggregate sample as follows:
Table 5
| Počet obalů v partii | Počet dílčích vzorků |
|---|---|
| 1 - 10 | dílčí vzorek z každého obalu |
| 11 - 30 | dílčí vzorky z každého třetího obalu, nejméně však z 10 obalů |
| nad 31 | dílčí vzorky z každého pátého obalu, nejméně však z 10 obalů |
(2) From a lot of seed in packages weighing 30 kg or less, packaging shall be assembled in such a way as to produce sampling units of approximately 100 kg. Each sampling unit shall be considered as one package, followed in accordance with paragraph 1.
(3) Partial samples shall be taken from bulk seed from different depths depending on the weight of the lot.
Table 6
| Hmotnost partie | Počet dílčích vzorků |
|---|---|
| do 1 tuny | 5 dílčích vzorků |
| nad 1 tunu | 10 dílčích vzorků |
(4) Partial samples shall be taken by hand or by hand in a large container where they are thoroughly mixed.
(5) For garlic seed, the average sample shall be compiled directly from the incremental samples.
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Regulation Information
| Citation | Decree No. 156 / 2021 Coll., amending Decree No. 61 / 2011 Coll., on the requirements for sampling, procedures and methods for testing seed and seed, as amended |
|---|---|
| Regulation Type | Order |
| Author | - |
| Collection | Code of Laws |
| Date of Promulgation | 08.04.2021 |
|---|---|
| Effective from | 15.04.2021 |
| Effective until | - |
| Status | Valid |
Legal Areas:
Administrative law
Agriculture
The regulation text is for informational purposes only.
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