Decree No. 400 / 2004 Coll.
Decree amending Decree No. 275 / 1998 of the Ministry of Agriculture Coll., on agrochemical testing of agricultural soils and determining the soil properties of forest land, as amended by Decree No. 477 / 2000 Coll.
Valid
Order
Effective from 01.08.2004
Text versions:
01.08.2004
07.07.2004
400
DECLARATION
of 23 June 2004
amending Decree No. 275 / 1998 of the Ministry of Agriculture Coll., on agrochemical testing of agricultural soils and the detection of the soil properties of forest parcels, as amended by Decree No. 477 / 2000 Coll.
The Ministry of Agriculture provides in the agreement with the Ministry of Health pursuant to Sections 10 (1) and (8) and 16 (d) of Act No. 156 / 1998 Coll., on fertilisers, soil improvers, aids and substrates and on agrochemical testing of agricultural soils (Fertilisers Act), as amended by Act No. 317 / 2004 Coll.:
Decree No. 275 / 1998 Coll., on agrochemical testing of agricultural soils and soil characteristics of forest parcels, as amended by Decree No. 477 / 2000 Coll., is amended as follows:
1. In Article 2, the following paragraph 5 is added:
"(5) In cases provided for in the fertiliser law, agrochemical testing of agricultural soils shall also include microbiological and physical analyses. ';
2. In Article 3, paragraphs 4 and 5 are added:
"(4) The principles of microbiological analysis of agricultural soils and the criteria for evaluating their results are set out in Annex 7.
(5) The principles of physical analysis of agricultural soils and the criteria for assessing their results are set out in Annex 8. '
3. In Annex 3, the following points 4 to 6 are inserted after point 3:
"4. Determination of oxidable carbon
Determination procedure: Oxidable organic carbon in soil is oxidized with chromium acid in an environment of excess sulphuric acid under defined conditions. The results expressed in% Cox can be converted into% humus provided that the humic acids contain 58% C.
5. Determination of total nitrogen
Method of determination: The soil sample is mineralized by wet concentrated sulphuric acid in the presence of a catalyst (Kjeldahl method) in which the organic compounds N are oxidized to NH4 +, which is determined after distillation by neutralising the excess of acid consumed by the volumetric solution of the principle.
6. Determination of potential cation exchange capacity
Method of determination: The soil sorbent complex is saturated with barium ions by repeated extraction with barium chloride solution adjusted to pH 8,1. In the eluate, acidimetric titration determines the exchange hydrogen. In the second step, sorbed barium from the sample is extracted with magnesium chloride solution. The second eluate shall determine the barium concentration directly proportional to the potential cation exchange capacity. ';
4. the following Annexes 7 and 8 are inserted after Annex 6:
"Annex No 7 to Decree No. 275 / 1998 Coll.
Microbiological analyses of agricultural soils
1. Principles of microbial analysis of agricultural soils
Determination of abundance and soil microflora activity by measuring respiratory curves (ISO DIS 17155)
Fresh soil samples with a water content equal to 40-60% of maximum water capacity are pre-incubated for 3-4 days at constant temperature before starting the measurement. The recommended temperature ranges from 20 to 25 ° C. During measurements, carbon dioxide released or oxygen consumed is determined at regular intervals (recommended every hour). Basal respiration rate is defined as the amount of CO2 released or O2 consumed per unit of time without adding a substrate. Respiratory curves are measured after addition of an easily degradable substrate (e.g. glucose). The measurements are carried out until the respiration rate is reduced. Any device allowing continuous measurement of released carbon dioxide or oxygen may be used to measure respiration.
The parameters characterising biomass and the activity of the microbial community are subtracted from the respiratory curves: a substrate induced by respiration, lag phase, growth rate and time tpeak. The respiration-induced substrate is understood as a constant increase in respiratory rate immediately after the addition of the substrate, lag phase time until the onset of exponential growth in respiratory rate and growth rate of the rate constant measured during the exponential growth of respiratory rate. Tpeak time is the time from the addition of the substrate to the maximum respiratory rate. The respiration activation quotient QR is calculated from the basis and substrate-induced respiration.
Nitrification potential - rapid test by measuring the rate of oxidation of ammonium ions (ISO DIS 15685)
The oxidation rate of ammonium ions to nitrite is measured within 6 hours of incubation in soil suspension buffered to pH 7.2. The substrate is added to the suspension in the form of ammonium sulphate. Oxidation of nitrites to nitrates is inhibited by the addition of sodium chloride. Nitrogen ions are determined by an appropriate analytical method.
In order to determine whether soil contains a contaminant that affects nitrification, a soil control sample with a known nitrification potential of 500-800 ng N.g-1.h-1. shall be used. Samples of test and control soil are pre-incubated for two days at moisture equivalent to water content at 60% of maximum water capacity. A composite sample of 1: 1 is then prepared (converted into dry soil). The composite sample and soil samples tested and checked are incubated for 1 day at 20 ° C and then the nitrification potential is determined.
2. Evaluation criteria
Determination of abundance and soil microflora activity by measuring respiratory curves
Contaminated soils show higher QR values and longer lag phase time and tpeak max. QR > 0.3 or phase lag > 20 h and tpeak > 50h at QR 0.2-0.3 indicate contaminated soil.
Nitrification potential - quick test by measuring the rate of oxidation of ammonium ions
The test soil is considered to be contaminated if the nitrification potential of the composite sample is less than 90% of the mean nitrification potential of the test and control soil samples determined separately.
As + SD < 0.9 * Avop
As - activity of the composite sample
Aprum - activity calculated as mean of control and test soil
SD - Standard deviation.
Příloha č. 8
Annex No 8 to Decree No. 275 / 1998 Coll.
Physical analyses of agricultural soils
Determination of granular composition.
Determination procedure: Soil particles are divided by size into granular fractions. The basic division of the skeleton from the soft ground is the dimension of 2 mm which was chosen because it is the upper limit of capillary water movement. Sedimentation pipette method is most commonly used for grading grain fractions. It consists of a small proportion being collected from certain depths of sedimentation suspension in the sedimentation cylinder after the respective periods, which is weighed after drying. The results of the granular analysis are generally adjusted to the table.
Assessment criteria: For agronomic purposes, the Earth classification scale according to Novák is used, which classifies the soil according to the fraction representation below 0,01 mm.
| Obsah částic (zrn) menších 0, 01 mm v % | Označení druhu půdy | Klasifikace půdy | |
| 0 – 10 | písčitá | P | lehká |
| 10 – 20 | hlinitopísčitá | HP | lehká |
| 20 – 30 | písčitohlinitá | PH | středně těžká |
| 30 – 45 | hlinitá | H | středně těžká |
| 45 – 60 | jílovitohlinitá | JH | těžká |
| 60 – 75 | jílovitá | JV | těžká |
| přes 75 | jíl (nebo prchlice) | J | těžká |
Determination of skeleton contents.
Method of determination: The volume of the sample shall be determined by the water in the volumetric cylinder, which shall be divided by the sieves per fraction, the volume of which shall be gradually determined again in the graduated cylinder. Result - the volume of individual fractions is expressed in% of the total sample volume.
Assessment criteria: Classification scale, created for comprehensive soil survey, divides soil into fine soil (under 2 mm) and skeleton. It consists of three fractions (rough sand 2-4 mm, gravel 4- 30 mm, stones above 30 mm). The range of application is suitable for larger samples (in kg order) taken exclusively for this determination.
Skeletons in the ornice up to 20% generally do not affect plant growth or cultivation. The higher volume is at the expense of a fine country, and the layers of solid clay skeleton are particularly unfavourable.
Determination of physical properties.
Method of determination: Physical characteristics mean the indicators, which are collected on an intact soil sample, taken into a metal ring. The procedure is suitable for fine-grained cohesive soil without gross skeleton or large organic residues. For the sample, preferably taken from the probe, the mass of fresh, water-saturated, drained and dried samples shall be determined and its apparent density shall be given the basic data for the calculation of the indicators sought: the volume humidity of the instantaneous (instantaneous, original), the maximum capillary water capacity, the instantaneous (original) airspeed, the minimum air capacity, the moisture content, the volume mass reduced (dry), the porosity.
The evaluation criteria reflect the properties of the soil. The current humidity (volume%) and the current airspeed (volume%) depend on the particular situation and climate. Maximum capillary water capacity (volume%) - the rougher the soil, the smaller. For fine-grained, heavy and light soils, it is larger (it is usually 20 - 40%). Minimum air capacity (volume%) - below 10% for field soils and below 5% for meadow soils - vulnerable to contamination, if permanent - contaminated, above 20% for fields - dry, dry, dry, dry. Suction (volume%) - indicates saturation of the entire soil column, the so-called capillary pores. For clay soils it is higher than for sand soils. Based on the volume weight of dry soil and the porosity, we can approximately evaluate the structural state of the humus horizon of medium and heavy soils (Kutílek, 1966):
| Strukturní stav humusového horizontu | Objemová hmotnost suché půdy g . cm-3 | Pórovitost % |
| výborný | méně než 1,2 | více než 54 |
| dobrý | 1,2 – 1,4 | 46 - 54 |
| nevyhovující | 1,4 – 1,6 | 39 - 46 |
| nestrukturní | 1,6 – 1,8 | 31 – 39.“. |
Efficacy
This Decree shall take effect on 1 August 2004.
Minister:
Ing. Palas v. r.
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Regulation Information
| Citation | Decree No. 400 / 2004 Coll., amending Decree No. 275 / 1998 of the Ministry of Agriculture Coll., on agrochemical testing of agricultural soils and detection of soil properties of forest parcels, as amended by Decree No. 477 / 2000 Coll. |
|---|---|
| Regulation Type | Order |
| Author | - |
| Collection | Code of Laws |
| Date of Promulgation | 07.07.2004 |
|---|---|
| Effective from | 01.08.2004 |
| Effective until | - |
| Status | Valid |
The regulation text is for informational purposes only.
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