Decree No. 187 / 2005 Coll.
Decree amending Decree No. 252 / 2004 Coll., laying down sanitary requirements for drinking and hot water and the frequency and scope of control of drinking water
Valid
Order
Effective from 01.06.2005
Text versions:
01.06.2005
18.05.2005
187
DECLARATION
of 4 May 2005
amending Decree No. 252 / 2004 Coll., laying down sanitary requirements for drinking and hot water and the frequency and scope of control of drinking water
The Ministry of Health provides, pursuant to § 108 (1) of Act No. 258 / 2000 Coll., on the Protection of Public Health and on the Amendment of Certain Related Acts, as amended by Act No. 320 / 2002 Coll. and Act No. 274 / 2003 Coll., for the implementation of § 3 (1), (3) and (5) and § 4 (1), (4) and (7) and § 41a (1) of the Act and pursuant to § 19 (1) (a), (b) and (f) of Act No. 110 / 1997 Coll., on Food and Tobacco Products and on the amendment and addition of certain related Acts, as amended by Act No. 316 / 2004 Coll.:
Decree No. 252 / 2004 Coll., laying down the hygiene requirements for drinking and hot water and the frequency and scope of control of drinking water, is amended as follows:
1. In Article 3, the sentence "Drinking water must have physical and chemical characteristics which do not pose a threat to public health shall be inserted at the beginning of paragraph 1. '
2. In Article 3 (2), the words "first and second sentences' shall be inserted after the words" Article 3 (3) '.
3. In Article 3 (3), the words "paragraph 1 'shall be inserted after the words" Article 41a'.
4. In Article 4, the following paragraph 5 is added:
"(5) The control of water used in means of transport shall be carried out within the scope of a reduced analysis, extended where appropriate by indicators, the value of which may be changed by means of materials with which water comes into contact, or by additional treatment of water, provided that the water is taken from the tap where the water quality complies with the requirements of this Decree. ';
5. In the second sentence of Article 5 (3), the words "microcystin LR," shall be inserted after the words "cyanide,"
6. In Article 7 (2), first and second sentences, the words "Annexes 1 and 2 'are replaced by the words" Annexes 1 to 3'.
7. in the second sentence of Paragraph 8 (1) (c), "limit values" is replaced by "limit values."
8. In Article 9 (2), the words "non-compliance with the health limits laid down 'are replaced by the words" effectiveness of corrective measures implemented'.
9. In the second sentence of Article 11 (1), the words "and fluoride and sulphate indicators for which stable values are considered to be satisfactory without abnormal changes with findings below 75% of the hygiene limit 'shall be inserted after the words" acceptable'.
10. In Annex No 1, Part A, in column 5 of the Explanatory Notes, the numbers "3, 4 'are replaced by" 3'.
11. In Annex 1, Part A indicator No 7 of the column Explanatory Notes is replaced by "3, 4, 5 '.
12. In Annex 1, Part A, indicator 8 reads:
| „8 | počty kolonií při 22 °C | KTJ/ml | 200 | MH | 6 |
| KTJ/ml | 100 | NMH | 2“. |
13. In Annex 1, Part A, indicator 9 reads:
| „9 | počty kolonií při 36 °C | KTJ/ml | 20 | MH | 7 |
| KTJ/ml | 20 | NMH | 2“. |
14. In Annex 1, Part B, indicator No 21 reads:
| „21 | bromičnany | BrO3 | μg/l | 10 | NMH | 11,35“. |
15. in Annex No 1 the explanatory notes to the tables in the last sentence of point 1 are replaced by "§ 3 (1)";
16. In Annex 1 to the Explanatory Notes to the tables, point 4 reads as follows:
"4. Organisms covered by this indicator are for the purpose of the decree the sinuses and all eukaryonic organisms (algae, protozoa, micromycites, swirlers, tubers, etc.). Bacteria (with the exception of sinuses) are given only in the word description but are not counted in the total number of organisms. The microscopic finding of the mass occurrence of organotrophic bacteria (more than 100 individuals / ml) should be considered as exceeding the MH of indicators 6 and 7 respectively. Products of iron bacteria metabolism are listed as abiosestone. '
17. In Annex 1 The explanatory notes to the tables in point 24 shall be added at the end of the first sentence "during a period where an increased incidence of sinuses can be expected '.
18. In Annex 2 to the Explanatory Notes to the tables, point 2 reads as follows:
'2. For hospitals and other health and accommodation facilities, the limit shall apply as a limit; for other objects, it shall be the recommended value to be pursued by means of technical measures. A maximum limit of 0 cfu / 100 ml shall be required for the separation of hospitals where patients with reduced immunity are hospitalised. '
19. In Annex 2, point 4 of the Explanatory Notes to the tables:
"4. It shall not apply to controlled shock disinfection, where higher doses of disinfectant may be used, provided that, by means of organisational measures, the water thus treated is not used for human consumption (drinking and bathing). ';
20. Annex 3, including the title:
"Annex 3 to Decree No. 252 / 2004 Coll.
Microbiological, biological, physical, chemical and organoleptic hot water indicators produced from an individual source for the personal hygiene of workers and their hygiene limits
A. Microbiological requirements
| Ukazatel | Jednotka | Limit | Vysvětlivky |
|---|---|---|---|
| atypická mykobakteria | KTJ/1000 ml | 100 | 1,3 |
| Escherichia coli | KTJ/100 ml | 0 | |
| legionely | KTJ/100 ml | 100 | 2,3 |
| počet kolonií při 36 °C | KTJ/1 ml | 200 | |
| Pseudomonas aeruginosa | KTJ/100 ml | 0 | |
| Staphylococcus aureus | KTJ/100 ml | 0 |
B. Physical and chemical requirements
| Ukazatel | Jednotka | Limit | Vysvětlivky |
|---|---|---|---|
| chemická spotřeba kyslíku (manganistanem) | mg/l | 5,0 | |
| pach | přijatelný pro odběratele | 4 | |
| pH | 6 – 9,5 | ||
| trihalometany | μg/l | 100 | |
| volný chlor | mg/l | 0,1 – 1,0 | 5 |
| vizuální posouzení | 6 | ||
| zákal | ZF(t,n) | 5 | 7 |
Explanatory notes:
1. The limit applies to the sum of the following species of atypical mycobacteria: Mycobacterium chelonae, M. kansasii, M. avium, M. intracellulare, M. crophulaceum, M. xenopi, M. fortunitum.
2. The Legionel test need not be carried out when heating is carried out at the point of consumption (e.g. by a flow heater).
3. Indicators shall be determined only in the case of the production of hot water from a source of surface water or mine water and with central heating and distribution.
4. In case of doubt, steps 1 and 2 shall be considered acceptable in the determination according to EN 1622. Determination of the odour threshold number (TON) and taste threshold number (TFN).
5. The determination shall be carried out at the sampling point. The content of free and bound chlorine or other disinfectant is determined only when substances containing chlorine are used in water treatment.
6. E.g. absence of coating or foam on the surface, colour etc.
7. The result shall be reported in accordance with the method used to determine: F (t) or F (n) where (t) means turbidimetric and (n) non-phelometric. If the limit value is exceeded, the cause must be immediately examined and effective corrective measures taken. ';
21. In Annex 4, Part A, the following sentence is added to the Explanatory Notes to Table *: "Where the number of people supplied, e.g. in the light of seasonal recreation, varies significantly and cannot be clearly identified, the average volume of water produced (m3 / day) shall be taken as a basis for determining the frequency for the annual period."
22. In Annex 6, Part A, the following row is inserted in the table under the indicator "Clostridium perfringens (including the dispute) ':" atypical mycobacteria' and in the column "See the procedure below '.
23. In Annex 6, Part A, the state of Clostridium perfringens (including the dispute) is renumbered as point 1 and the following point 2 is added:
"2. Atypical mycobacteria:
One of the following methods may be used to determine atypical mycobacteria:
(a) Sodium lauryl sulphate method:
Procedure for establishing:
The membrane filter (0,45 μm porosity filter) is processed using a water pump of 0,5 l water. Under sterile conditions, the filter is removed with tweezers and scissor cut to 6-8 strips, which are transferred to a centrifugation container with 20 ml sterile distilled water and shake for 10 minutes on the shaker. The solution is then poured into another container and centrifuged for 20 minutes at 2000 g to 3000 g. Dispose of the supernatant and decontaminate the sediment with 3 ml of 3% sodium lauryl sulphate with 1% NaOH. After 20 minutes of shaking, add 25 ml of sterile distilled water and centrifuge again at 2000 g to 3000 g for 20 minutes. After casting of the supernatant, approximately 2 ml of the saline solution is added to the sediment so that the resulting sample volume in the test tube is aligned to a certain value which will serve to calculate the quantity. After homogenisation with pipette, the sample should be vaccinated in a quantity of 0,25 ml per six egg soils (3 Löwenstein-Jensen and 3 Ogawa soils). Two of the vaccinated soils (1 L.-J. and 1 Ogawa) are incubated at 30 ° C, 37 ° C and 42 ° C. The result shall be deducted after 1, 3, 6 and 9 weeks, the results after 9 weeks of incubation shall be used to determine the numbers. If the strain does not grow at a certain temperature, the results are calculated only from those incubation temperatures at which the strain is able to grow (some mycobacteria do not grow at below 37 ° C). Their morphology and pigmentation shall be recorded in grown colonies and their acidoresistance shall be verified in microscopic terms with ZiehlNeelsen dye. In case of positivity, species identification of isolated strain shall be carried out in specialised mycobacteriological laboratories.
The number of mycobacteria per litre of water shall be calculated in relation to the volume of filtered water, the volume of the sample after centrifugation, the number of vaccinated fences and the size of the inoculum vaccinated.
Chlorhexidine may also be used to decontaminate water. Add 10 ml of 0,2% chlorhexidine aqueous solution to the sediment, shake manually and leave it at room temperature for 24 hours. The second day after centrifugation and after washing the sediment 10 ml of sterile distilled water, the sediment shall be treated after further centrifugation at 2000 g to 3000 g as with sodium lauryl sulphate.
(b) Cetylpyridinium chloride (CPC) method:
Procedure for establishing:
The membrane filter (0,45 μm porosity filter) is treated with a water pump of 1 litre of water. For the decontamination of the water sample, the CPC solution shall be used at the resulting concentration of 0,005% to be prepared for sterile distilled water.
Decontamination of the water sample is performed as follows: 1 part of 0,05% of the CPC solution is added to 9 parts of the water sample. After closing the bottle, the contents are shaken for 30 seconds, then left to stand for 30 minutes. After that, filtration shall be performed immediately through a cellulose membrane filter with a diameter of 50 mm and a pore size of 0,45 μm.
The filter is rinsed after completion of filtration of 500 ml of sterile distilled water to remove the residua CPC.
Remove the filter with sterile tweezers and insert into a sterile 30 ml plastic container containing 2 ml of sterile distilled water. On the vortex, the plastic container is shaken for 10 seconds. On the surface of the soil in Petri dishes, the suspension is inoculated with 0,2 ml and 0,1 ml is used to paint on the slide for coloring using the ZiehlNeelsen method. Cultivation on one petri dish with Löwenstein-Jensen soil and one petri dish with ogawa soil at 30 ° C, 37 ° C and 42 ° C. Evaluation shall be performed once a week within 9 weeks of incubation.
The report shall record the results found on individual soils: number of colonies on the soil, massive growth on the section with a representative increase, morphology of colonies, microscopic verification of grown colonies. If more mycobacterial species grow, the number of each species shall be reported separately. 10 colonies are collected for identification, from which a solid-soil subculture (Löwenstein-Jensen or Ogawa) is prepared to carry out identification tests. In some suspected colonies of mycobacteria, it is possible to test the gene Probe directly from the primoculture from a sufficient inoculum. The final growth assessment is performed after 9 weeks of incubation, as well as the conclusion of negative tests (for negative incubation it will be extended to 12 weeks). If during the six-week incubation, all inoculated soil exceeds the non-specific bacterial flora, the result shall be closed earlier.
For the determination of the numbers, the results shall be used after 9 weeks of incubation (or 12 weeks of incubation if negative testing was carried out at 9 weeks). If the strain does not grow at a certain temperature, the results are calculated only from those incubation temperatures at which the strain is able to grow. The number of colonies of mycobacteria per litre of water shall be calculated in relation to the volume of filtered water, the sample volume when the filter is shaken, the size of the inoculum vaccinated and the number of inoculated fences. ';
24. In Annex 6, Part B, the explanatory notes to the table in point 2 read:
'2. Accuracy is the tightness of conformity between independent test results obtained under pre-specified conditions. Accuracy expresses the degree of variance of observation around the mean. Accuracy depends only on the distribution of random errors and does not relate to the actual value. The opinion on accuracy may be related to any set of measurements carried out under given experimental conditions that can be selected arbitrarily (repeatability, reproducibility). The accuracy of the measurement set may be expressed quantitatively by one of the sampling characteristics of the variance, such as the sampling standard deviation. ';
This Decree shall take effect on 1 June 2005.
Minister:
Doc. MUDr. Emmer, CSc.
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Regulation Information
| Citation | Decree No. 187 / 2005 Coll., amending Decree No. 252 / 2004 Coll., laying down the hygiene requirements for drinking and hot water and the frequency and scope of control of drinking water |
|---|---|
| Regulation Type | Order |
| Author | - |
| Collection | Code of Laws |
| Date of Promulgation | 18.05.2005 |
|---|---|
| Effective from | 01.06.2005 |
| Effective until | - |
| Status | Valid |
The regulation text is for informational purposes only.
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